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KMID : 0370220110550030267
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Yakhak Hoeji
2011 Volume.55 No. 3 p.267 ~ p.272
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Anticancer Effect of Activated Natural Killer Cells on Human Non-small Cell Lung Cancer
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Park Min-Gyeong
Sung Hye-Ran
Park Ji-Sung
Kim Jee-Youn
Han Sang-Bae
Lee Chong-Kil
Yun Byung-Kui
Song Suk-Gil
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Abstract
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Human NK cells, identified 30 years ago based on their ability to spontaneously kill tumor cells, constitute a subset of lymphocytes, which play an important role in the first line of immune defense and the effective function of these cells are enhanced by cytokines. Lung carcinoma has been one of the most commonly diagonosed cancer as well as the leading cause of cancer death in male. Here we provide the evidence that human natural killer cells has inhibitory effects on tumor growth of human lung cancer cell NCI-H460 (non-small cell lung cancer). Enriched NK cell population was obtained by 2 weeks cultivation in interleukin-2(IL-2)-containing medium. The resulting population comprised 26% CD3+ cells, 9% CD3+CD4+ cells, 16% CD3+CD8+ cells, 76% CD56+ cells, 6% CD3+CD56+ cells and 70% CD3-CD56+ cells. Activated NK cells at doese of 2.5, 5, and 10 million cells per mouse inhibited 2%, 12% and 45% of NCI-H460-induced tumor growth in nude mouse xenograft assays, repectively. This result suggests that NK cell-based immunotherapy may be used as an adoptive immunotherapy for lung cancer patients.
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KEYWORD
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natural killer cells, immunotherapy, NCI-H460 lung cancer
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